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ICSI (Intra Cytoplasmic Sperm Injection)

Intra Cytoplasmic Sperm Injection which is more popularly referred to as ICSI treatment is basically a in vitro fertilisation procedure. In this procedure, a single sperm cell is injected into the cytoplasm of a female egg directly where the acrosome reaction is skipped or avoided. After the fertilisation is successful, the embryo is allowed to grow for a few days and then it is finally transferred into the uterus or the womb of the females. At KARE, we have trained and vastly experienced practitioners who make sure that the procedure of ICSI is successful. Moreover, the infertility treatment procedure of Intra Cytoplasmic Sperm Injection is readily preferred because of the minimised chances of failure.

Why ICSI is Needed?

Intra Cytoplasmic Sperm Injection or ICSI is a very efficient and effective infertility treatment procedure which is important to treat the following problems listed below.

  • The male partner is unable to produce the required sperm count for carrying out the procedures of intrauterine insemination or in vitro fertilisation.
  • The sperms are immobile and are unable to move in the normal way.
  • The sperms have trouble in attaching with the egg and fertilising it.
  • Any kinds of blockages in the reproductive tract of the males thereby preventing the sperms from getting out.
  • Eggs are not being fertilised with the help of traditional in vitro fertilisation irrespective of the condition of the sperm.
  • ICSI is helpful if in vitro matured eggs or previously frozen eggs are being used.

We at KARE, very well understand the importance of ICSI in overcoming infertility and hence we prefer its use to a significant extent.

Procedure of Treatment

There are few necessary steps followed for carrying out the procedure of Intra Cytoplasmic Sperm Injection.

Preparation for ICSI

Here the females are treated with hormonal drugs which helps in the stimulation of their ovaries for the production of multiple matured eggs so that the chances of creation of a number of viable embryos are higher. This is turn also enhances the chances of maintaining capability for pregnancy.

Removal of the Egg

In this step, the matured egg is retrieved from the ovaries of the females with the help of a transvaginal needle which is guided by ultrasound. The egg is retrieved and placed in a petri dish for the purpose of fertilisation.

Collection of Semen

The next step in ICSI procedure involves collection of semen from the males. If the sperm count is low in an ejaculation, then a fine needle is used for the extraction of the sperm. Even if there is insufficiency, then a biopsy is taken from the tissue of the testicles for getting sperm.


In the next step, Intra Cytoplasmic Sperm Injection is performed under the guidance of a microscope where a single sperm is inserted directly into the cytoplasm of the egg with the aim of fertilisation. The experts at KARE very carefully control the fertilisation process and after fertilisation the embryos are transferred into a special incubator.

Monitoring the Embryo’s Development

About 48 hours after fertilisation, rapid cell division occurs which are carefully monitored and then the specialists at KARE select the healthiest of the embryos for the purpose of transferring eggs to the uterus of the females.

Transferring of the Embryo

This is the final step of ICSI where the embryo is transferred into the uterus or the womb of the females and results are checked after two weeks for a successful pregnancy. Our specialists make sure to perform routine check - up and ultrasound for the appropriate monitoring of the pregnancy.

Authored by Dr. Nirmal Krishnan

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